Recognition of forked and single‐stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks
Copyright policy )Recognition of forked and single‐stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks
Post‐replication DNA repair facilitates the resumption of DNA synthesis upon replication fork stalling at DNA damage sites. Despite the importance of RAD18 and polymerase η (Polη) for post‐replication repair (PRR), the molecular mechanisms by which these factors are recruited to stalled replication forks are not well understood. We present evidence that human RAD18 complexed with RAD6B protein preferentially binds to forked and single‐stranded DNA (ssDNA) structures, which are known to be localized at stalled replication forks. The SAP domain of RAD18 (residues 248–282) is crucial for binding of RAD18 complexed with RAD6B to DNA substrates. RAD18 mutated in the SAP domain fails to accumulate at DNA damage sites in vivo and does not guide DNA Polη to stalled replication forks. The SAP domain is also required for the efficient mono‐ubiquitination of PCNA. The SAP domain mutant fails to suppress the ultraviolet (UV)‐sensitivity of Rad18‐knockout cells. These results suggest that RAD18 complexed with RAD6B is recruited to stalled replication forks via interactions with forked DNA or long ssDNA structures, a process that is required for initiating PRR.
- Kyushu University Japan
- Japan Science and Technology Agency Japan
- Osaka University Japan
- Kumamoto University Japan
DNA Replication, Binding Sites, Base Sequence, DNA Repair, Ultraviolet Rays, Ubiquitin-Protein Ligases, Ubiquitination, DNA, Single-Stranded, DNA-Directed DNA Polymerase, Protein Structure, Tertiary, DNA-Binding Proteins, Multiprotein Complexes, Proliferating Cell Nuclear Antigen, Ubiquitin-Conjugating Enzymes, Humans, DNA Damage
DNA Replication, Binding Sites, Base Sequence, DNA Repair, Ultraviolet Rays, Ubiquitin-Protein Ligases, Ubiquitination, DNA, Single-Stranded, DNA-Directed DNA Polymerase, Protein Structure, Tertiary, DNA-Binding Proteins, Multiprotein Complexes, Proliferating Cell Nuclear Antigen, Ubiquitin-Conjugating Enzymes, Humans, DNA Damage
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