Neotropiellais a genus of springtails which can be of medium size (2 mm) or relatively long (5 mm). These springtails live in leaf litter, under the bark of dead trees or in decomposing wood, mainly in the Neotropical Region and are often collected by litter samples on Berlese funnels or by pitfall traps. Most species have been described, based on relatively few specimens and chaetotaxy of several species is incomplete.A new species withinNeotropiellawas discovered in recent pitfall trap collections from Peru.Neotropiella peruanasp. n. was taxonomically treated and studied under both phase contrast and scanning electron microscopy. It is similar toN. insularisfrom Brazil, but smaller with only 4 mandibular teeth (vs. 5) and with well-developed unguis lateral teeth. Intraspecific variation of the new species is provided. We also present the first DNA barcodes for the genus.