TTLL10 is a protein polyglycylase that can modify nucleosome assembly protein 1
Copyright policy )TTLL10 is a protein polyglycylase that can modify nucleosome assembly protein 1
Certain proteins can undergo polyglycylation and polyglutamylation. Polyglutamylases (glutamate ligases) have recently been identified in a family of tubulin tyrosine ligase‐like (TTLL) proteins. However, no polyglycylase (glycine ligase) has yet been reported. Here we identify a polyglycylase in the TTLL proteins by using an anti‐poly‐glycine antibody. The antibody reacted with a cytoplasmic 60‐kDa protein that accumulated in elongating spermatids. Using tandem mass spectrometry of trypsinized samples, immunoprecipitated by the antibody from the TTLL10‐expressing cells, we identified the 60‐kDa protein as nucleosome assembly protein 1 (NAP1). Recombinant TTLL10 incorporated glycine into recombinant NAP1 in vitro. Mutational analyses indicated that Glu residues at 359 and 360 in the C‐terminal part of NAP1 are putative sites for the modification. Thus, TTLL10 is a polyglycylase for NAP1.
- University of California System United States
- Hamamatsu University School of Medicine Japan
- Mitsubishi Japan
- University of California, Irvine United States
- University of California, San Francisco United States
Male, Spermiogenesis, Molecular Sequence Data, Glutamic Acid, Cell Cycle Proteins, Antibodies, Mice, Animals, Amino Acid Sequence, Peptide Synthases, Glycylation, Glycylase, Nucleosome Assembly Protein 1, Nuclear Proteins, Tubulin tyrosine ligase, Immunohistochemistry, Spermatids, Mice, Inbred C57BL, Histone, Polyglutamic Acid, Nucleosome assembly protein, Carrier Proteins, Protein Processing, Post-Translational
Male, Spermiogenesis, Molecular Sequence Data, Glutamic Acid, Cell Cycle Proteins, Antibodies, Mice, Animals, Amino Acid Sequence, Peptide Synthases, Glycylation, Glycylase, Nucleosome Assembly Protein 1, Nuclear Proteins, Tubulin tyrosine ligase, Immunohistochemistry, Spermatids, Mice, Inbred C57BL, Histone, Polyglutamic Acid, Nucleosome assembly protein, Carrier Proteins, Protein Processing, Post-Translational
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