Cloning and characterization of E‐dlg, a novel splice variant of mouse homologue of the Drosophila discs large tumor suppressor binds preferentially to SAP102
Copyright policy )Cloning and characterization of E‐dlg, a novel splice variant of mouse homologue of the Drosophila discs large tumor suppressor binds preferentially to SAP102
AbstractMembrane‐associated guanylate kinases (MAGUKs) act as scaffolds to coordinate signaling events through their multiple domains at the plasma membrane. The MAGUK SH3 domain is noncanonical and its function remains unclear. To identify potential binding partners of MAGUK SH3, the synapse‐associated protein 102 (SAP102) SH3 domain was used as bait in a yeast two‐hybrid screen of a mouse embryonic cDNA library. A mouse homologue of the Drosophila discs large tumor suppressor (Dlg, also known as SAP97) bound preferentially to SAP102 SH3. The 4347bp cDNA sequence encoded an 893 amino acid protein with 94% identity to mouse SAP97. A deleted region (33‐aa) strongly suggests this is a novel splice variant, which we call Embryonic‐dlg/SAP97 (E‐dlg). The interaction of SAP102 and E‐dlg was confirmed in mammalian cells. E‐dlg can also bind to potassium channel Kv1.4 in a pull‐down assay. E‐dlg was highly expressed in embryonic and some adult mouse tissues, such as brain, kidney, and ovary. Furthermore, in situ hybridization showed that E‐dlg was mostly expressed in olfactory bulb and cerebellum. © 2008 IUBMB IUBMB Life, 60(10): 684–692, 2008
- Hokkaido Bunkyo University Japan
- Johns Hopkins University United States
- Johns Hopkins Medicine United States
- Hokkaido University Japan
- Johns Hopkins University School of Medicine United States
Recombinant Fusion Proteins, Molecular Sequence Data, Neuropeptides, Membrane Proteins, Olfactory Bulb, Discs Large Homolog 1 Protein, Mice, Inbred C57BL, Alternative Splicing, Mice, Cerebellum, Animals, Drosophila Proteins, Humans, Protein Isoforms, Genes, Tumor Suppressor, Amino Acid Sequence, Guanylate Kinases, Cells, Cultured, Adaptor Proteins, Signal Transducing, Protein Binding
Recombinant Fusion Proteins, Molecular Sequence Data, Neuropeptides, Membrane Proteins, Olfactory Bulb, Discs Large Homolog 1 Protein, Mice, Inbred C57BL, Alternative Splicing, Mice, Cerebellum, Animals, Drosophila Proteins, Humans, Protein Isoforms, Genes, Tumor Suppressor, Amino Acid Sequence, Guanylate Kinases, Cells, Cultured, Adaptor Proteins, Signal Transducing, Protein Binding
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).2 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!