NAT, a Human Complex Containing Srb Polypeptides that Functions as a Negative Regulator of Activated Transcription
Copyright policy )NAT, a Human Complex Containing Srb Polypeptides that Functions as a Negative Regulator of Activated Transcription
A complex that represses activated transcription and contains the human homologs of the yeast Srb7, Srb10, Srb11, Rgr1, and Med6 proteins was isolated. The complex is devoid of the Srb polypeptides previously shown to be components of the yeast Mediator complex that functions in transcriptional activation. The complex phosphorylates the CTD of RNA polymerase II (RNAPII) at residues other than those phosphorylated by the kinase of TFIIH. Moreover, the complex specifically interacts with RNAPII. The interaction is not mediated by the CTD of RNAPII, but is precluded by phosphorylation of the CTD. Our results indicate that the complex is a subcomplex of the human RNAPII holoenzyme. We suggest that the RNAPII holoenzyme is a transcriptional control panel, integrating and responding to specific signals to activate or repress transcription.
- University of Medicine and Dentistry of New Jersey United States
- Howard Hughes Medical Institute United States
- Harvard University United States
Transcriptional Activation, DNA, Complementary, Mediator Complex, Saccharomyces cerevisiae Proteins, Base Sequence, Sequence Homology, Amino Acid, Macromolecular Substances, Molecular Sequence Data, Cell Biology, Cyclin-Dependent Kinases, Fungal Proteins, Repressor Proteins, Cyclins, Trans-Activators, Animals, Humans, Amino Acid Sequence, RNA Polymerase II, Phosphorylation, Molecular Biology, Transcription Factors
Transcriptional Activation, DNA, Complementary, Mediator Complex, Saccharomyces cerevisiae Proteins, Base Sequence, Sequence Homology, Amino Acid, Macromolecular Substances, Molecular Sequence Data, Cell Biology, Cyclin-Dependent Kinases, Fungal Proteins, Repressor Proteins, Cyclins, Trans-Activators, Animals, Humans, Amino Acid Sequence, RNA Polymerase II, Phosphorylation, Molecular Biology, Transcription Factors
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).209 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!