Mouse Germline Restriction of Oct4 Expression by Germ Cell Nuclear Factor
Copyright policy )Mouse Germline Restriction of Oct4 Expression by Germ Cell Nuclear Factor
The POU-domain transcription factor Oct4 is essential for the maintenance of the mammalian germline. In this study, we show that the germ cell nuclear factor (GCNF), an orphan nuclear receptor, represses Oct4 gene activity by specifically binding within the proximal promoter. GCNF expression inversely correlates with Oct4 expression in differentiating embryonal cells. GCNF overexpression in embryonal cells represses Oct4 gene and transgene activities, and we establish a link to transcriptional corepressors mediating repression by GCNF. In GCNF-deficient mouse embryos, Oct4 expression is no longer restricted to the germ cell lineage after gastrulation. Our studies suggest that GCNF is critical in repressing Oct4 gene activity as pluripotent stem cells differentiate and in confining Oct4 expression to the germline.
- Baylor College of Medicine United States
- Justus Liebig University Giessen Germany
- University of Pennsylvania United States
- Centre national de la recherche scientifique France
Homeodomain Proteins, Mice, Knockout, Macromolecular Substances, Fushi Tarazu Transcription Factors, Gene Expression Regulation, Developmental, Nuclear Proteins, Antineoplastic Agents, Cell Differentiation, Embryo, Mammalian, Cell Line, DNA-Binding Proteins, Mice, Germ Cells, Gene Expression Regulation, Genes, Reporter, Nuclear Receptor Subfamily 6, Group A, Member 1, Animals, Nuclear Receptor Co-Repressor 1, Cell Lineage, In Situ Hybridization, Developmental Biology
Homeodomain Proteins, Mice, Knockout, Macromolecular Substances, Fushi Tarazu Transcription Factors, Gene Expression Regulation, Developmental, Nuclear Proteins, Antineoplastic Agents, Cell Differentiation, Embryo, Mammalian, Cell Line, DNA-Binding Proteins, Mice, Germ Cells, Gene Expression Regulation, Genes, Reporter, Nuclear Receptor Subfamily 6, Group A, Member 1, Animals, Nuclear Receptor Co-Repressor 1, Cell Lineage, In Situ Hybridization, Developmental Biology
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).220 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!