RAD21 and KIAA0196 at 8q24 are amplified and overexpressed in prostate cancer
Copyright policy )RAD21 and KIAA0196 at 8q24 are amplified and overexpressed in prostate cancer
AbstractTo detect genes that are overexpressed in prostate cancer, a subtracted cDNA library was first constructed from the PC‐3 cell line and subsequently screened by using cDNA microarray hybridization. Sixty‐eight genes were found to be overexpressed (ratio > 3) in PC‐3. Half of these genes were in chromosomal regions, which, using comparative genomic hybridization, we previously showed to be gained in PC‐3. Subsequently, the expression and copy number of selected genes were studied by quantitative RT‐PCR and fluorescence in situ hybridization in prostate cancer cell lines, xenografts, and clinical tumor specimens of benign prostate hyperplasia and untreated as well as hormone‐refractory prostate carcinomas. Two genes from chromosomal region 8q24—RAD21 and KIAA0196—showed increased expression in clinical prostate carcinomas and were also amplified in 30–40% of xenografts and hormone‐refractory tumors. In addition, the expression of KIAA0196 was significantly (P = 0.0051) higher in tumors with the gene amplification than in those without it. The data suggest that KIAA0196 and possibly RAD21 are putative target genes for the common amplification of 8q23‐24 in prostate cancer. © 2003 Wiley‐Liss, Inc.
- University of Washington United States
- Tampere University Hospital Finland
- University of Mary United States
- Tampere University Finland
Male, DNA, Complementary, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Gene Amplification, Gene Dosage, Nuclear Proteins, Nucleic Acid Hybridization, Prostatic Neoplasms, Cell Cycle Proteins, Blotting, Northern, Phosphoproteins, DNA-Binding Proteins, Gene Expression Regulation, Neoplastic, Cell Line, Tumor, Humans, In Situ Hybridization, Fluorescence, Chromosomes, Human, Pair 8, Genes, Neoplasm, Oligonucleotide Array Sequence Analysis
Male, DNA, Complementary, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Gene Amplification, Gene Dosage, Nuclear Proteins, Nucleic Acid Hybridization, Prostatic Neoplasms, Cell Cycle Proteins, Blotting, Northern, Phosphoproteins, DNA-Binding Proteins, Gene Expression Regulation, Neoplastic, Cell Line, Tumor, Humans, In Situ Hybridization, Fluorescence, Chromosomes, Human, Pair 8, Genes, Neoplasm, Oligonucleotide Array Sequence Analysis
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