The Biosphere Reserve "Askania Nova" Reports is a scientific, peer-reviewed journal specialized in biology and ecology. Thematic scope: the journal publishes articles on nature conservation, conservation of natural diversity, biology, ecology, protection of rare species of plants and animals in nature and artificial conditions, steppe nature management, soil science, introduction, reintroduction. Issues: protected area, bioecology; genetics, plant physiology and applied botany; hydrobiology; zoology. Periodicity: 1 time a year. The dataset contains an records of all species occurrences that have been published in 2-25 issues of the Biosphere Reserve "Askania Nova" Reports (2000-2024). To avoid data duplication, this dataset does not include the occurrences that are part of another dataset (https://doi.org/10.15468/4gzpkr, https://doi.org/10.15468/nhn2ae, https://doi.org/10.15468/xyt6hj) published before. This dataset contains 5007 taxa names recorded. The nomenclature of the dataset follows original taxa names in articles. The materials in the Biosphere Reserve "Askania Nova" Reports are especially important in light of the events of 2022, when a Russian full scale invasion. The data from these Scientific readings actually document the historical state of biodiversity before the outbreak of a full-scale invasion war in the southern Ukraine.