Melanoma is the most invasive skin cancer with the highest risk of death. Better understanding of the pathogenesis of melanoma has helped identify new therapeutic targets and drugs against melanoma in prolonging the survival of melanoma patients. Ginkgetin is a bioflavonoid compound extracted from the leaves of Ginkgo biloba, which exhibits a multitude of biological activities such as antiviral, antioxidant, anti-inflammatory, and antifungal. Although it has been shown to suppress the progression of multiple types of tumors, its role in melanoma remains unexplored. Therefore, we investigated the role and potential mechanism of ginkgetin in melanoma. The results of the studies reported here show that ginkgetin inhibits proliferation and promotes apoptosis of the melanoma cell lines. Ginkgetin also induces G1/G0 arrest of the melanoma cells by regulating signal transducer and activator of transcription 3 and mitogen-activated protein kinase signaling pathways. We therefore believe that ginkgetin may be a promising drug in the treatment and cure of melanoma.