Let us ponder how remarkable is our body’s architecture, how from single cells we become a complex organism. During embryonic development, cells need to multiply, differentiate, change shape, and move to ultimately create our body architecture. A key question that drives my dissertation work is: how can epithelial cells change shape and move while maintaining their organization and integrity? Proper tissue organization requires the cell-cell adherens junctions (AJ) to be connected to the actomyosin cytoskeleton, as well as cell polarity establishment and maintenance. I investigated the role and mechanism of action of Canoe, an AJ-cytoskeletal linker, during Drosophila development. The overarching theme emerging from my work is that the machinery mediating AJ-cytoskeleton linkage and apical-basal polarity establishment does not form a simple linear pathway. Instead, a complex multivalent network of protein, in which each interaction has different degrees of importance, with some essential while other dispensable, is involved.First, I investigated how the small GTPase Rap1 regulates Canoe during apical-basal polarity establishment. I found that Canoe’s cortical localization during polarity establishment is regulated by active Rap1, which binds Canoe’s RA domains. Strikingly, Canoe’s RA domains are dispensable for membrane localization, but critical for apical restriction and tricellular junction (TCJ) enrichment. Dizzy, a Rap1 activator, is essential for Canoe’s TCJ enrichment.Second, I investigated how Canoe works as an AJ-cytoskeletal linker ensuring tissue robustness under mechanical tension. Our data revealed that during germband elongation and dorsal closure Canoe ivreinforces junctions under tension and in its absence Polychaetoid plays a parallel role. Embryos lacking both Canoe and Polychaetoid have early junctional failure leads to loss of tissue integrity.Finally, I defined how Canoe works as a machine, given that Canoe’s multiple domains are largely unexplored in vivo. I discovered that Canoe’s PDZ and F-actin binding domains are dispensable for viability, and for Canoe AJ localization and TCJ enrichment. The F-actin binding domain reinforces junction under mechanical tension. However, Canoe’s RA domains are important for its TCJ and vertical border enrichment during planar polarity, which are areas of high tension. My work reinforces the idea that multiple inputs ensure tissue integrity during development.