This database compiles occurrences of different taxa of marine and freshwater invertebrate species recorded from 2008 to 2023 in the Subantarctic regions, including the Magellanic region, the Cape Horn Reserve Biosphere, the sub-Antarctic islands of South Georgia and Kerguelen, the west and east coasts of the Antarctic Peninsula, the South Shetland Islands, and the South Orkney Islands. This database is the result of six different research projects: 1. (Metadata ID: marine-invertebrates I): Project Title: Differences in the evolutionary success of three genera of Antarctic Littorinidae: an approach from historical biogeography. Principal investigator: Sebastián Rosenfeld. 2. (Metadata ID: marine-invertebrates II): Project Title: "Filogeografia e historia evolutiva de la especie Neobuccinum eatoni (Mollusca, Neogastropoda) en el océano Austral" and "Cryptic speciation In the Southern ocean: integrating genomics, morphology and species distribution models to elucidate the speciation process of a gastropod group". Principal investigator: Angie Díaz. 3. (Metadata ID: Intertidal-quironomid-I-RBCH): Project Title: Ecophysiology, Morphology and Phylogeography of Insects in the Scotia Arc. Principal investigator: Felipe Simoes. 4. (Metadata ID: freshwater-invertebrates I): Project Title: Benthic macroinvertebrates of temperate, subantarctic streams: the effects of altitudinal zoning and temperature on the phenology of aquatic insects associated to the Róbalo river, Navarino island (55°S), Chile. Principal investigator: Tamara Contador. 5.(Metadata ID: freshwater-invertebrates II): Project Title: Ecological features of subantarctic aquatic insects: integrating approaches to assess southern Chile freshwater biodiversity. Principal investigator: Javier Rendoll. 6. (Metadata ID: Branchinecta-gaini): Project Title: Range changes triggered by global warming in the fairy shrimp branchinecta gaini: new insights. Principal investigator: Claudia Maturana.