A dataset contains 130,054 unique occurrences of 1,465 vascular plant taxa (species, hybrids, aggregates) from Vladimir Oblast (Russia) and tiny parts of adjacent areas. They are largely based on field studies by Alexey P. Seregin performed in 1999–2020 (121,737 records) as well as on data extracted from relevant literature, manuscripts, herbarium collections, and citizen science projects (8,317 records). The most important collections from Vladimir Oblast are deposited in the Moscow University herbarium (MW), the Komarov Institute herbarium in St. Petersburg (LE), and Murom Art Museum. Georeferences are based on WGS84 grid scheme with 342 squares ranging from 94.7 to 98.2 square km (5.0 minutes lat. × 10.0 minutes long.). Dataset contains only one occurrence per species per grid square, thereby earlier records are not duplicated if they were confirmed recently. Each occurrence is placed in the grid square centroid, therefore real coordinates, habitat details and voucher information are unavailable. The dataset was used to produce grid maps in the “Flora of Vladimir Oblast: checklist and atlas” (Seregin et al. 2012). Additional records resulted from field excursions of 2012 and 2013 were fully covered in “Flora of Vladimir Oblast: grid data analysis” (Seregin 2014), the second monograph of the series – is an analytical part of the survey with quantitative diversity assessment given. Data obtained in field in 2014-2020 as well as extracted from recently published souces, were published in April 2021. The last update added 7,000 new records.