This database gathers 10,721 specimens from vascular flora (angiosperms, gymnosperms, and pteridophytes) deposited in the Herbarium of the Pontificia Universidad Católica de Valparaíso (PUCV) en Chile. This botanical collection was started by the renowned botanist Otto Zöllner and represents a major natural history legacy for Central Chile, with decades of information represented through preserved specimens. This collection is currently deposited in the Curauma campus of the PUCV. This digitization effort is part of the PUCV to mobilize its biological collections and make them freely available through GBIF, encouraging national and international researchers to generate new knowledge based on this invaluable heritage, which is a silent witness of the vast plant diversity that once existed in Chile and that is now vanishing due to anthropogenic drivers.