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БИОЭЛЕКТРОКАТАЛИТИЧЕСКИЕ СВОЙСТВА БАКТЕРИАЛЬНЫХ ДВУХДОМЕННЫХ ЛАККАЗ

BIOELECTROCATALYTIC PROPERTIES OF BACTERIAL TWO-DOMAIN LACCASES

БИОЭЛЕКТРОКАТАЛИТИЧЕСКИЕ СВОЙСТВА БАКТЕРИАЛЬНЫХ ДВУХДОМЕННЫХ ЛАККАЗ

Abstract

Методом амперометрии исследована возможность прямого переноса электронов в биоэлектрокаталитических системах на основе двухдоменных лакказ: полученных путем сайт-направленного мутагенза лакказы бактерий Streptomyces carpinensis ВКМ Ас-1300 и лакказы Catenuloplanes japonicus ВКМ Ас-875. Доля прямого переноса электронов на кислород у лакказы Catenuloplanes japonicus ВКМ Ас-875 составила 75%. Выполненный биоинформационный анализ показал новые возможности для прогнозирования определенных характеристик новых лакказ, необходимых для их направленного взаимодействия с поверхностью электродов.

The possibility of direct electron transfer in bioelectrocatalytic systems based on two-domain laccases, obtained by site-directed mutagenization of bacterial Streptomyces carpinensis VKM Ac-1300 laccase and Catenuloplanes japonicus VKM Ac-875 laccase, was studied by amperometry. The proportion of direct electron transfer to oxygen in the laccase Catenuloplanes japonicus VKM Ac-875 was 75%. The performed bioinformatic analysis showed new possibilities for predicting certain characteristics of new laccases necessary for their directed interaction with the electrode surface.

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Keywords

БИОКАТОД, biocathode, БАКТЕРИАЛЬНЫЕ ДВУХДОМЕННЫЕ ЛАККАЗЫ, bioelectrocatalysis, ПРЯМОЙ ПЕРЕНОС ЭЛЕКТРОНОВ, МЕДИАТОРНЫЙ ПЕРЕНОС ЭЛЕКТРОНОВ, mediated electron transfer, direct electron transfer, bacterial two-domain laccases, БИОЭЛЕКТРОКАТАЛИЗ

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average