The dataset contains records of the Macrophytes (i.e., aquatic and riparian plants) collected in the lower valley of the River Tua and its tributaries, and included in the Ecological Monitoring Program (2011-2015) of the Foz Tua Hydroelectric Dam, promoted by EDP – Energias de Portugal, S.A. Data were collected in the spring 2011-2015 during field visits to 17 sampling points distributed along the valley of river Tua (Northeast Portugal) and its tributaries, up to the intersection with rivers Rabaçal and Tuela, 52 kms upstream. These data are part of a larger monitoring program in the river Tua basin to evaluate the impacts of the dam construction in the water quality of the river, before and during the construction process.