The main objective of this paper is to present the Herbarium of the Pontifical Catholic University of Parana's and it collection. The history of the HUCP’s collection began in the middle 1970s with the foundation of the Biology Museum. This museum gathered both botanical and Zoological specimens. Only in April, 1979 collections where separated and HUCP was founded with preserved specimens of algae (green, red and brown), fungi, lichen and embryophytes. On October, 2016, it encompasses nearly 25,000 specimens from at about 4,000-4,500 species, 1,600 genera and 297 families. Most of the specimens comes from the state of Paraná but it also has specimens from many Brazilian states and other countries, mainly from south America (Chile, Argentina, Uruguay, Paraguay and Colombia) but also from other parts of the world (Cuba, USA, Spain, Germany, China and Australia). Our collection includes 42 fungi and lichen, 122 gimnosperms, 305 briophytes, 2765 pteridophytes, 3065 algae and 18.000 angiosperms and only one type (Mimosa tucumensis Barneby ex Ribas, M. Morales & Santos-Silva – Fabaceae). We also have botanical and education for sustainability programs for basic and high school students and training for teachers. https://www.pucpr.br/escola-de-ciencias-da-vida/infraestrutura/herbario/ HUCP data are free for non commercial use. Users must give appropriate credits, provide a link to the collection at SIBBr, indicate if changes were made, cite original Data Paper (http://dx.doi.org/10.3390/data2010010), inform the use and send a copy of publications to Curator. Researcher and institutions are responsible for adequate uses of the data. The HUCP herbaria did no guarantee correct identification of specimens or associated data. Data Paper reference: Kersten, R.A., Salesbram, J. A. M. & Acra, L.A. 2017. Herbarium of the Pontifical Catholic University of Paraná (HUCP), Curitiba, Southern Brazil. Data 2017, 2(1), 10.