Ferns from the family Ophioglossaceae kept in two herbarium collections (NS and NSK) placed at the Digital herbarium of the Central Siberian Botanical Garden, Siberian Branch of the Russian Academy of Sciences are represented in this dataset. The moonwort genus, Botrychium s. s., includes diploid and polyploid taxa that occur primarily in the northern hemisphere. Their evolutionary history, morphologically cryptic taxa and deep divergence of the family in the phylogeny of ferns has long fascinated pteridologists.