Soil macrofauna communities were evaluated in a number of land use systems on a private farm (Estância JAE) in Santo Inácio, Paraná, Brazil. Sampling was performed in October 2013 and January 2014 in seven land uses: 1) an agrosilvopastoral system with Eucalyptus urograndis tree rows and Urochloa ruziziensis pasture and annual crops (soybean, maize, oats, wheat) planted in the interrow; 2) a silvopastoral system with rows of Corymbia maculata trees and the interrow planted with Urochloa ruziziensis; 3) a permanent pasture of Urochloa sp.; 4) a sugarcane plantation with conventional tillage; 5) an agropastoral system with soybean in the summer and Urochloa ruziziensis pasture in the winter; 6) a 20-year old Eucalyptus sp. plantation; and 7) a native Atlantic Forest fragment, nearby a stream. Samples were taken using the standard methodology of the Tropical Soil Biology and Fertility Programme (TSBF), where soil (down to 20 cm depth) and litter fauna were hand-sorted from monoliths of 25x25 cm, and the abundance of a total of 42 taxa was assessed.