A dataset contains 5,503 unique occurrences of ca. 500 vascular plant species from Pereslavsky and Rostovsky Districts of Yaroslavl Oblast (Russia). They are based on field records by Alena G. Frontova performed in 2014 using a grid scheme. Georeferences are based on WGS84 grid scheme with 31 grid squares. Field recording was performed within a small grid square (2,5′ lat. x 5′ long.). Only one small grid square (SW, SE, NW, or NE) per larger one (5′ lat. x 10′ long.) was sampled. Occurrence is placed in the small grid square centroid. Dataset contains only one occurrence per species per grid square. The dataset is a part of the Bachelor's Paper "Flora of the southern tip of Yaroslavl Oblast" (Frontova, 2015) supervised by Dr. A.P. Seregin. Voucher specimens were trasfered to the Moscow University Herbarium and are available via the "Moscow University Herbarium (MW)" dataset (https://www.gbif.org/dataset/902c8fe7-8f38-45b0-854e-c324fed36303).