These data were collected by teachers and pupils of the biological educational center from Moscow (http://www.dnttm.su/) and researchers of the Institute of mathematical problems in biology from Pucshino. The teachers have high qualification in ornithology and flora studies. Study area is located on the right bank of the Klyazma River about 100 km East of Moscow. Our dataset is first biodiversity data for this location available through GBIF.org. Study site is covered by forests and flood-meadows; the majority of forest is dominated by oak (Quercus robur); the meadows have a rich plant species diversity. The occurrences were found during route studies, coordinates were fixed by GPS garmin 64s. The total length of the routes was about 10 km. We identified 69 bird and 117 plant species. Among them some protected species in Vladimir region. There are Iris sibirica, Galium intermedium, Haematopus ostralegus, Locustella naevia and Bubo bubo.