Because benthic macroinvertebrates and zooplankton are susceptible to the pesticide rotenone, we surveyed freshwater macroinvertebrates in the Miller Creek Watershed, Kenai Peninsula, Alaska ahead of a rotenone treatment in fall 2021 to eradicate a population of invasive northern pike (Esox lucius Linnaeus, 1758). We collected 32 samples in 2021 and another 32 post-treatment invertebrate samples in 2022 at the same places and during the same time of year to enable comparison of pre- and post-treatment freshwater invertebrate communities.