Biogenesis of the protein import channel Tom40 of the mitochondrial outer membrane: intermembrane space components are involved in an early stage of the assembly pathway.
Copyright policy )Biogenesis of the protein import channel Tom40 of the mitochondrial outer membrane: intermembrane space components are involved in an early stage of the assembly pathway.
Tom40 forms the central channel of the preprotein translocase of the mitochondrial outer membrane (TOM complex). The precursor of Tom40 is encoded in the nucleus, synthesized in the cytosol, and imported into mitochondria via a multi-step assembly pathway that involves the mature TOM complex and the sorting and assembly machinery of the outer membrane (SAM complex). We report that opening of the mitochondrial intermembrane space by swelling blocks the assembly pathway of the beta-barrel protein Tom40. Mitochondria with defects in small Tim proteins of the intermembrane space are impaired in the Tom40 assembly pathway. Swelling as well as defects in the small Tim proteins inhibit an early stage of the Tom40 import pathway that is needed for formation of a Tom40-SAM intermediate. We propose that the biogenesis pathway of beta-barrel proteins of the outer mitochondrial membrane not only requires TOM and SAM components, but also involves components of the intermembrane space.
- University of Freiburg Germany
- La Trobe University Australia
Saccharomyces cerevisiae Proteins, 060100 Biochemistry and Cell Biology, Membrane Proteins, Membrane Transport Proteins, Intracellular Membranes, Mitochondrial Membrane Transport Proteins, Mitochondria, Mitochondrial Proteins, Protein Transport, 0601 (four-digit-FOR), Protein Precursors, Mitochondrial Swelling, 060108 Protein Trafficking
Saccharomyces cerevisiae Proteins, 060100 Biochemistry and Cell Biology, Membrane Proteins, Membrane Transport Proteins, Intracellular Membranes, Mitochondrial Membrane Transport Proteins, Mitochondria, Mitochondrial Proteins, Protein Transport, 0601 (four-digit-FOR), Protein Precursors, Mitochondrial Swelling, 060108 Protein Trafficking
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