Additional file 2: Table S1. Assessment of sema.460 target site conservation. The 21 bp sequence targeted by sema.460 was used as a blastn query against all Aedes, Anopheles and Culex genomes in Vectorbase. Mosquito species bearing perfectly conserved target sequences are listed; the gene numbers (if known) or scaffold locations (s) corresponding to each match are indicated. The sema.460 target sequence was also used as an input for NCBI blastn searches conducted on the indicated taxonomic groups; corresponding taxonomic identification numbers (TaxIDs) are listed for each group. As of December 2018, searches against all sequences in the blast database did not uncover any perfect matches to the sema.460 target sequence outside of the indicated disease vector mosquito species.