The paper provides the updated checklist of the genus Hypena Schrank from Korea. This genus is one of the largest genera within the Noctuoidea comprising more than 680 species worldwide and the genus is the monophyletic group based on the morphological characters. The external examination along with the genitalia examination and DNA barcoding could reveal the diversity of the genus in Korea. In this study, we examined a total of 192 specimens and barcoded 16 species and listed a total of 29 species of Hypena including five new additions, Hypena tamsi Filipjev (1927), Hypena obacerralis Walker (1859), Hypena pulverulenta Wileman (1911), Hypena perspicua Leech (1900) and Hypena mandarina Leech (1900) to the Korean fauna. We provided the detailed distribution of each species of the genus across South Korea and the photographs of adults and genitalia. In addition, the monophyly of the genus was also confirmed using two outgroup species of Herminiinae. This study significantly contributes to the knowledge of erebid fauna in Korea and the phylogenetic relationship amongst the species of the genus.