Broad Specificity in Binding of NIPP-1, Nuclear Inhibitor of Protein Phosphatase-1, to PP1 Isoforms in Vivo
Copyright policy )Broad Specificity in Binding of NIPP-1, Nuclear Inhibitor of Protein Phosphatase-1, to PP1 Isoforms in Vivo
Protein phosphatase type-1 (PP1), one of the most abundant Ser/Thr protein phosphatases, plays a central role in the regulation of various cell functions. Almost all the PP1 molecules exist as holoenzymes in vivo consisting of a catalytic subunit (PP1C) and a variable regulatory subunit that regulates substrate specificity and/or subcellular localization. In order to clarify fine regulation of PP1, we overexpressed a nuclear inhibitor of PP1 (NIPP-1) in a Flag-tagged form in mammalian cells. The Flag-tagged NIPP-1 was found to be immunoprecipitated with three isoforms of PP1C, namely, PP1alpha, PP1gamma1, and PP1delta with a similar efficiency, suggesting that NIPP-1 makes a complex with the PP1C through the region conserved among the three isoforms. These results suggested that NIPP-1 can be involved in the regulation of various PP1 holoenzymes in vivo.
- Hokkaido Bunkyo University Japan
- Hokkaido University Japan
Recombinant Fusion Proteins, Intracellular Signaling Peptides and Proteins, RNA-Binding Proteins, Cell Line, Isoenzymes, Protein Phosphatase 1, COS Cells, Endoribonucleases, Phosphoprotein Phosphatases, Animals, Humans, Enzyme Inhibitors, Carrier Proteins, Protein Binding
Recombinant Fusion Proteins, Intracellular Signaling Peptides and Proteins, RNA-Binding Proteins, Cell Line, Isoenzymes, Protein Phosphatase 1, COS Cells, Endoribonucleases, Phosphoprotein Phosphatases, Animals, Humans, Enzyme Inhibitors, Carrier Proteins, Protein Binding
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