Abstract Stevens, W.D., J.-N. Labat & F. Barthelat (2016). Two new species of Apocynaceae, Asclepiadoideae from Mayotte. Candollea 71:127–134. In English, English and French abstracts. DOI : http://dx.doi.org/10.15553/c2016v711a15 Two new species endemic to Mayotte are described and illustrated : Marsdenia mayottae W.D. Stevens, Labat & Barthelat and Tylophora mayottae W.D. Stevens, Labat & Barthelat (Apocynaceae, Asclepiadoideae). Marsdenia mayottae is similar to Marsdenia vohiborensis Choux from Madagascar but differs by its very small or sometimes absent corona. Tylophora mayottae is morphologically close to Tylophora coriacea Marais but differs by its much smaller flowers. These two new species, discovered in during recent inventories, are assessed as threaten following the Categories and the Criteria of the IUCN Red List.