Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation
Copyright policy )Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation
The Saccharomyces cerevisiae gene RRP1 encodes an essential, evolutionarily conserved protein necsessary for biogenesis of 60S ribosomal subunits. Processing of 27S pre-ribosomal RNA to mature 25S rRNA is blocked and 60S subunits are deficient in the temperature-sensitive rrp1-1 mutant. We have used recent advances in proteomic analysis to examine in more detail the function of Rrp1p in ribosome biogenesis. We show that Rrp1p is a nucleolar protein associated with several distinct 66S pre-ribosomal particles. These pre-ribosomes contain ribosomal proteins plus at least 28 nonribosomal proteins necessary for production of 60S ribosomal subunits. Inactivation of Rrp1p inhibits processing of 27SA3 to 27SBS pre-rRNA and of 27SB pre-rRNA to 7S plus 25.5S pre-rRNA. Thus, in the rrp1-1 mutant, 66S pre-ribosomal particles accumulate that contain 27SA3 and 27SBL pre-ribosomal RNAs.
- Carnegie Mellon University United States
Ribosomal Proteins, Saccharomyces cerevisiae Proteins, Molecular Sequence Data, Nuclear Proteins, Saccharomyces cerevisiae, RNA, Ribosomal, FOS: Biological sciences, RNA Precursors, Amino Acid Sequence, Ribosomes, Sequence Alignment, Cell Nucleolus, 69999 Biological Sciences not elsewhere classified
Ribosomal Proteins, Saccharomyces cerevisiae Proteins, Molecular Sequence Data, Nuclear Proteins, Saccharomyces cerevisiae, RNA, Ribosomal, FOS: Biological sciences, RNA Precursors, Amino Acid Sequence, Ribosomes, Sequence Alignment, Cell Nucleolus, 69999 Biological Sciences not elsewhere classified
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