Regulation of cellular plasticity inDrosophilaimaginal disc cells by the Polycomb group, trithorax group andlamagenes
Copyright policy )Regulation of cellular plasticity inDrosophilaimaginal disc cells by the Polycomb group, trithorax group andlamagenes
Drosophila imaginal disc cells can switch fates by transdetermining from one determined state to another. We analyzed the expression profiles of cells induced by ectopic Wingless expression to transdetermine from leg to wing by dissecting transdetermined cells and hybridizing probes generated by linear RNA amplification to DNA microarrays. Changes in expression levels implicated a number of genes: lamina ancestor, CG12534 (a gene orthologous to mouse augmenter of liver regeneration), Notch pathway members, and the Polycomb and trithorax groups of chromatin regulators. Functional tests revealed that transdetermination was significantly affected in mutants for lama and seven different PcG and trxG genes. These results validate our methods for expression profiling as a way to analyze developmental programs, and show that modifications to chromatin structure are key to changes in cell fate. Our findings are likely to be relevant to the mechanisms that lead to disease when homologs of Wingless are expressed at abnormal levels and to the manifestation of pluripotency of stem cells.
- Washington State University United States
- University of California, San Francisco United States
- University of Mary United States
- University of California System United States
Polycomb Repressive Complex 1, Gene Expression Profiling, Gene Expression Regulation, Developmental, Embryonic Structures, Nerve Tissue Proteins, DNA-Binding Proteins, Drosophila melanogaster, Multigene Family, Morphogenesis, Animals, Drosophila Proteins, In Situ Hybridization, Oligonucleotide Array Sequence Analysis, Transcription Factors
Polycomb Repressive Complex 1, Gene Expression Profiling, Gene Expression Regulation, Developmental, Embryonic Structures, Nerve Tissue Proteins, DNA-Binding Proteins, Drosophila melanogaster, Multigene Family, Morphogenesis, Animals, Drosophila Proteins, In Situ Hybridization, Oligonucleotide Array Sequence Analysis, Transcription Factors
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