Inhibition of Recombining Binding Protein Suppressor of Hairless (RBPJ) Impairs the Growth of Prostate Cancer
Copyright policy )Inhibition of Recombining Binding Protein Suppressor of Hairless (RBPJ) Impairs the Growth of Prostate Cancer
Background/Aims: Notch signaling pathway regulates cancer cell growth. RBPJ is a key transcription factor downstream of Notch receptor activation, whereas the role of RBPJ in carcinogenesis of prostate cancer is ill-defined. Methods: Here, we evaluated the effects of RBPJ inhibition on the growth of prostate cancer cells. We knocked down RBPJ in prostate cancer cells by a short hairpin interfering RNA (shRNA). We measured cell growth by an MTT assay. We analyzed the levels of cell-cycle-associated proteins by Western blot. Results: We found that shRNA for RBPJ efficiently inhibited RBPJ expression in prostate cancer cells, resulting in a significant decrease in the cell growth. Further, RBPJ-mediated cell-growth inhibition appeared to be resulting from alteration of cell-cycle inhibitors p21 and p27, cell-cycle activators CDK2, CDK4 and CyclinD1, and apoptosis-suppressor Bcl-2. Conclusion: Our data suggest that shRNA intervention of RBPJ expression could be a promising therapeutic approach for treating human prostate cancer.
- Xi’an Jiaotong-Liverpool University China (People's Republic of)
- Xi'an Jiaotong University China (People's Republic of)
Male, Prostate cancer, Physiology, Prostatic Neoplasms, QD415-436, RBPJ, Biochemistry, Notch signaling pathway, Cancer growth, HEK293 Cells, Cell Line, Tumor, Immunoglobulin J Recombination Signal Sequence-Binding Protein, QP1-981, Humans, RNA, Small Interfering, Cell Proliferation
Male, Prostate cancer, Physiology, Prostatic Neoplasms, QD415-436, RBPJ, Biochemistry, Notch signaling pathway, Cancer growth, HEK293 Cells, Cell Line, Tumor, Immunoglobulin J Recombination Signal Sequence-Binding Protein, QP1-981, Humans, RNA, Small Interfering, Cell Proliferation
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