A Strategy for Probing the Function of Noncoding RNAs Finds a Repressor of NFAT
Copyright policy )A Strategy for Probing the Function of Noncoding RNAs Finds a Repressor of NFAT
Noncoding RNA molecules (ncRNAs) have been implicated in numerous biological processes including transcriptional regulation and the modulation of protein function. Yet, in spite of the apparent abundance of ncRNA, little is known about the biological role of the projected thousands of ncRNA genes present in the human genome. To facilitate functional analysis of these RNAs, we have created an arrayed library of short hairpin RNAs (shRNAs) directed against 512 evolutionarily conserved putative ncRNAs and, via cell-based assays, we have begun to determine their roles in cellular pathways. Using this system, we have identified an ncRNA repressor of the nuclear factor of activated T cells (NFAT), which interacts with multiple proteins including members of the importin-beta superfamily and likely functions as a specific regulator of NFAT nuclear trafficking.
- Scripps Research Institute United States
- Genomics Institute of the Novartis Research Foundation United States
RNA, Untranslated, NFATC Transcription Factors, Nuclear Proteins, beta Karyopherins, Cell Line, DNA-Binding Proteins, Mice, Animals, Humans, RNA Interference, RNA, Long Noncoding, Transcription Factors
RNA, Untranslated, NFATC Transcription Factors, Nuclear Proteins, beta Karyopherins, Cell Line, DNA-Binding Proteins, Mice, Animals, Humans, RNA Interference, RNA, Long Noncoding, Transcription Factors
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).675 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 0.1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!