IQ‐ArfGEF/BRAG1 is associated with synaptic ribbons in the mouse retina
Copyright policy )IQ‐ArfGEF/BRAG1 is associated with synaptic ribbons in the mouse retina
AbstractIQ‐ArfGEF/BRAG1 is a guanine nucleotide exchange factor for ADP ribosylation factors (Arfs), which are implicated in membrane trafficking and actin cytoskeleton dynamics. In this study, we examined the immunohistochemical localization of IQ‐ArfGEF/BRAG1 in the adult mouse retina using light and electron microscopy. IQ‐ArfGEF/BRAG1 was distributed in a punctate manner and colocalized well with RIBEYE in both the outer and inner plexiform layers. Immunoelectron microscopic analysis showed that IQ‐ArfGEF/BRAG1 was localized at the synaptic ribbons of photoreceptors. When heterologously expressed in HeLa cells, IQ‐ArfGEF/BRAG1 was recruited to RIBEYE‐containing clusters and formed an immunoprecipitable complex with RIBEYE. Furthermore, immunoprecipitation analysis showed that anti‐IQ‐ArfGEF/BRAG1 antibody efficiently pulled down RIBEYE from retinal lysates. These findings indicate that IQ‐ArfGEF/BRAG1 is a novel component of retinal synaptic ribbons and forms a protein complex with RIBEYE.
- Hokkaido University Japan
- Kitasato University Japan
- Hokkaido Bunkyo University Japan
Male, Intracellular Signaling Peptides and Proteins, Gene Expression, Membrane Proteins, Nerve Tissue Proteins, Phosphoproteins, DNA-Binding Proteins, Mice, Inbred C57BL, Alcohol Oxidoreductases, Mice, Microscopy, Electron, Transmission, Animals, Guanine Nucleotide Exchange Factors, Humans, Immunoprecipitation, Co-Repressor Proteins, Disks Large Homolog 4 Protein, Guanylate Kinases, HeLa Cells, Photoreceptor Cells, Vertebrate
Male, Intracellular Signaling Peptides and Proteins, Gene Expression, Membrane Proteins, Nerve Tissue Proteins, Phosphoproteins, DNA-Binding Proteins, Mice, Inbred C57BL, Alcohol Oxidoreductases, Mice, Microscopy, Electron, Transmission, Animals, Guanine Nucleotide Exchange Factors, Humans, Immunoprecipitation, Co-Repressor Proteins, Disks Large Homolog 4 Protein, Guanylate Kinases, HeLa Cells, Photoreceptor Cells, Vertebrate
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