Characterisation of a Chromatin Fraction Bearing Pulse-Labelled RNA. 1. Nascent RNA, RNA Polymerase B and Transcribed DNA Sequence Content
Copyright policy )Characterisation of a Chromatin Fraction Bearing Pulse-Labelled RNA. 1. Nascent RNA, RNA Polymerase B and Transcribed DNA Sequence Content
We have further characterised a fraction of polynucleosomal chromatin from mouse cells which is enriched in transcribing regions, after separation by multistep partition in a dextran /poly(ethylene glycol) two-phase mixture [Faber, A. J. (1972) Methods Cell Biol. 16, 447--457]. This fraction contains an average of 16% of the total DNA and 63% of the 3-min pulse-labelled RNA in chromatin which, by a number of criteria, represents nascent RNA transcripts. Of the total transcribing RNA polymerase B molecules in chromatin, 52% are found in this fraction by titration with radioactive alpha-amanitin. About 28% of the single-copy DNA sequences in this fraction hybridise to nuclear polyadenylated RNA, compared with only 1.5% of the single-copy sequences in the remaining (nontranscribing) chromatin fraction.
Mice, Base Sequence, Transcription, Genetic, Animals, Nucleic Acid Hybridization, RNA, DNA, RNA Polymerase II, Chromatin, Nucleosomes
Mice, Base Sequence, Transcription, Genetic, Animals, Nucleic Acid Hybridization, RNA, DNA, RNA Polymerase II, Chromatin, Nucleosomes
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