The TATA-binding protein participates in TFIIIB assembly on tRNA genes
Copyright policy )The TATA-binding protein participates in TFIIIB assembly on tRNA genes
The TATA-binding protein TBP has been recently recognized as a general class III transcription factor. Using the gel shift assay to monitor initiation complex assembly on a yeast tRNA gene, we show that TBP is required for the TFIIIC-dependent assembly of TFIIIB. TFIIIB depleted of TBP by a simple chromatographic step does not bind stably to the TFIIIC-tDNA complex. Addition of yeast or human recombinant TBP allows the formation of a TFIIIB-TBP-TFIIIC-tDNA complex. The presence of TBP in the complex was inferred from the effect of anti-TBP antibodies and from the different migration properties of TFIIIB-TBP-tDNA complexes formed with yeast or human TBP.
Transcription, Genetic, Genes, Fungal, Saccharomyces cerevisiae, TATA-Box Binding Protein, Recombinant Proteins, DNA-Binding Proteins, RNA, Transfer, Transcription Factor TFIIIB, Transcription Factors, TFIII, Transcription Factor TFIIIC, Humans, DNA, Fungal, Transcription Factors
Transcription, Genetic, Genes, Fungal, Saccharomyces cerevisiae, TATA-Box Binding Protein, Recombinant Proteins, DNA-Binding Proteins, RNA, Transfer, Transcription Factor TFIIIB, Transcription Factors, TFIII, Transcription Factor TFIIIC, Humans, DNA, Fungal, Transcription Factors
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