Abnormal V(D)J Recombination of T Cell Receptor β Locus in SMAR1 Transgenic Mice
Copyright policy )Abnormal V(D)J Recombination of T Cell Receptor β Locus in SMAR1 Transgenic Mice
Scaffold/matrix-associated region-1-binding protein (SMAR1) specifically interacts with the MARbeta sequence, which is located 400-bp upstream of the murine TCRbeta enhancer and is highly expressed during the DP stage of thymocyte development. To further analyze the functions of SMAR1, transgenic mice were generated that express SMAR1 in a tissue-independent manner. SMAR1-overexpressing mice exhibit severely altered frequency of the T cells expressing commonly used Vbetas (Vbeta5.1/5.2 and Vbeta8.1/8.2/8.3). The rearrangements of Vbeta5.1/5.2, Vbeta8.1/8.2/8.3 loci are also reduced in SMAR1 transgenic mice. The T cells in SMAR1 transgenic mice exhibit a mild perturbation at the early DN stage. SMAR1 transgenic mice exhibit hypercellular lymph nodes and spleen accompanied with prominent architectural defects in these organs. These results indicate that SMAR1 plays an important role in the regulation of T cell development as well as V(D)J recombination besides maintaining the architecture of the lymphoid organs.
- University of Pune India
- National Centre for Cell Science India
Reverse Transcriptase Polymerase Chain Reaction, T-Lymphocytes, Nuclear Proteins, Cell Cycle Proteins, Mice, Transgenic, DNA, Gene Rearrangement, T-Lymphocyte, DNA-Binding Proteins, Mice, Genes, T-Cell Receptor beta, Animals, Humans, Lymph Nodes, Promoter Regions, Genetic, VDJ Recombinases, Spleen, DNA Primers
Reverse Transcriptase Polymerase Chain Reaction, T-Lymphocytes, Nuclear Proteins, Cell Cycle Proteins, Mice, Transgenic, DNA, Gene Rearrangement, T-Lymphocyte, DNA-Binding Proteins, Mice, Genes, T-Cell Receptor beta, Animals, Humans, Lymph Nodes, Promoter Regions, Genetic, VDJ Recombinases, Spleen, DNA Primers
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