Accuracy of thymine–thymine dimer bypass by Saccharomyces cerevisiae DNA polymerase η
Copyright policy )Accuracy of thymine–thymine dimer bypass by Saccharomyces cerevisiae DNA polymerase η
The Saccharomyces cerevisiae RAD30 gene functions in error-free replication of UV-damaged DNA. RAD30 encodes a DNA polymerase, Pol η, which inserts two adenines opposite the two thymines of a cis - syn thymine–thymine (T–T) dimer. Here we use steady-state kinetics to determine the accuracy of DNA synthesis opposite the T–T dimer. Surprisingly, the accuracy of DNA synthesis opposite the damaged DNA is nearly indistinguishable from that opposite nondamaged DNA, with frequencies of misincorporation of about 10 −2 to 10 −3 . These studies support the hypothesis that unlike most DNA polymerases, Pol η is able to tolerate distortions in DNA resulting from damage, which then enables the polymerase to utilize the intrinsic base pairing ability of the T–T dimer.
- The University of Texas Medical Branch at Galveston United States
Base Sequence, DNA Polymerase iota, DNA-Directed DNA Polymerase, Templates, Genetic, Dimerization, Thymine, DNA Primers
Base Sequence, DNA Polymerase iota, DNA-Directed DNA Polymerase, Templates, Genetic, Dimerization, Thymine, DNA Primers
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