Functional reconstitution ofDrosophila melanogasterNMJ glutamate receptors
Copyright policy )Functional reconstitution ofDrosophila melanogasterNMJ glutamate receptors
SignificanceWe report the first functional reconstitution of neuromuscular (NMJ) glutamate receptors from the fruit flyDrosophila. The identification of these receptors enabled tremendous insight into the mechanisms of synapse assembly and development. However, analysis of animals with mutant receptors is complicated by compound phenotypes; studies on isolated receptors are necessary to identify the structural elements and auxiliary proteins important for receptor assembly, surface delivery, and function. We show that Neto is an essential component required for the function ofDrosophilaNMJ receptors expressed inXenopusoocytes, and use this system to examine subunit dependence and function. We find thatDrosophilaNMJ receptors have ligand-binding properties and structural features strikingly different from vertebrate glutamate receptors.
- National Institute of Health Pakistan
- National Institutes of Health United States
- National Institute of Child Health and Human Development United States
Ions, DNA, Complementary, Microscopy, Confocal, Cell Membrane, Molecular Sequence Data, Neuromuscular Junction, Membrane Proteins, Crystallography, X-Ray, Ligands, Permeability, Protein Structure, Tertiary, Drosophila melanogaster, Phenotype, Receptors, Glutamate, Mutation, Oocytes, Animals, Drosophila Proteins, Calcium, Amino Acid Sequence
Ions, DNA, Complementary, Microscopy, Confocal, Cell Membrane, Molecular Sequence Data, Neuromuscular Junction, Membrane Proteins, Crystallography, X-Ray, Ligands, Permeability, Protein Structure, Tertiary, Drosophila melanogaster, Phenotype, Receptors, Glutamate, Mutation, Oocytes, Animals, Drosophila Proteins, Calcium, Amino Acid Sequence
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