Toll-like receptor 2 contributes to liver injury by Salmonella infection through Fas ligand expression on NKT cells in mice
Copyright policy )Toll-like receptor 2 contributes to liver injury by Salmonella infection through Fas ligand expression on NKT cells in mice
Toll-like receptors (TLRs) for bacterial constitutes are expressed not only by phagocytes but also by some subsets of T cells. We previously reported that natural killer T cells (NKT cells) play an important role in liver injury induced by Salmonella infection. In the present study, we investigated whether TLRs on NKT cells are involved in Salmonella-induced liver injury.Gene expression of TLR2 was examined in sorted natural killer, NKT, and T cells from livers of naive mice by the reverse-transcription polymerase chain reaction method. Serum alanine aminotransferase level and FasL expression on liver lymphocytes were examined in TLR2-deficient (TLR2(-/-)) and FasL-deficient gld/gld mice before and after intraperitoneal inoculation of Salmonella choleraesuis 31N-1 using an enzyme-linked immunosorbent assay and flow cytometry.TLR2 gene was abundantly expressed by NKT cells freshly isolated from naive mice. FasL expression on liver NKT cells increased in TLR2(+/-) mice but not in TLR2(-/-) mice after Salmonella infection. Serum alanine aminotransferase level was significantly lower in the TLR2(-/-) and gld/gld mice than in the control mice after infection.TLR2 may contribute to liver injury induced by Salmonella infection via FasL induction on liver NKT cells.
- Nagoya University Japan
- Osaka University Japan
- Kanazawa University Japan
- Tokai National Higher Education and Research System Japan
Mice, Inbred C3H, Salmonella Infections, Animal, Fas Ligand Protein, Membrane Glycoproteins, T-Lymphocytes, Toll-Like Receptors, Gene Expression, Receptors, Cell Surface, Flow Cytometry, Mice, Mutant Strains, Toll-Like Receptor 2, Killer Cells, Natural, Mice, Inbred C57BL, Mice, Liver, Animals, Cytokines, Drosophila Proteins, Female
Mice, Inbred C3H, Salmonella Infections, Animal, Fas Ligand Protein, Membrane Glycoproteins, T-Lymphocytes, Toll-Like Receptors, Gene Expression, Receptors, Cell Surface, Flow Cytometry, Mice, Mutant Strains, Toll-Like Receptor 2, Killer Cells, Natural, Mice, Inbred C57BL, Mice, Liver, Animals, Cytokines, Drosophila Proteins, Female
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