Phosphorylation of a novel zinc-finger-like protein, ZPR9, by murine protein serine/threonine kinase 38 (MPK38)
Copyright policy )Phosphorylation of a novel zinc-finger-like protein, ZPR9, by murine protein serine/threonine kinase 38 (MPK38)
We have identified previously a new murine protein serine/threonine kinase, MPK38, closely related to the sucrose-non-fermenting protein kinase family [Gil, Yang, Lee, Choi and Ha (1997) Gene 195, 295–301]. Using the C-terminal half of the putative human counterpart of MPK38, HPK38, as a bait in a yeast two-hybrid screen of a human HeLa cDNA library, it was discovered that the zinc-finger-motif-containing protein, termed zinc-finger-like protein 9 (ZPR9), bound both HPK38 and MPK38. In a co-expression assay, ZPR9 associated with MPK38 in vivo, and we showed that the ZPR9 is also phosphorylated by MPK38. In addition, ZPR9 physically interacts with itself in mammalian cells. The ZPR9 cDNA hybridized with a mRNA species of approx. 1.7kb in Northern-blot analysis. The ZPR9 transcript was detected in all tissues examined, including lung, kidney, spleen, liver and brain. Co-expression of ZPR9 with MPK38 caused the accumulation of ZPR9 in the nucleus. These findings suggest a potentially important role for ZPR9 in MPK38-mediated signal transduction, and that ZPR9 is a physiological substrate of MPK38 in vivo.
- Chungbuk National University Korea (Republic of)
- Kyung Hee University Korea (Republic of)
- Pohang University of Science and Technology Korea (Republic of)
EXPRESSION, DNA, Complementary, yeast two-hybrid screen, Amino Acid Motifs, Molecular Sequence Data, NECROSIS-FACTOR RECEPTOR, substrate in vivo, Cell Line, Mice, CYTOPLASMIC DOMAIN, subcellular localization, BINDING, CD40, Animals, Humans, TRANSCRIPTION, Amino Acid Sequence, Cloning, Molecular, LEUCINE-ZIPPER, Glutathione Transferase, Cell Nucleus, Microscopy, Confocal, Base Sequence, RECOGNITION, Intracellular Signaling Peptides and Proteins, Nuclear Proteins, RING FINGER, Blotting, Northern, GENE, FAMILY, Microscopy, Fluorescence, association in vivo, SIGNALING PROTEINS, MOTIF, Carrier Proteins, Dimerization, HeLa Cells
EXPRESSION, DNA, Complementary, yeast two-hybrid screen, Amino Acid Motifs, Molecular Sequence Data, NECROSIS-FACTOR RECEPTOR, substrate in vivo, Cell Line, Mice, CYTOPLASMIC DOMAIN, subcellular localization, BINDING, CD40, Animals, Humans, TRANSCRIPTION, Amino Acid Sequence, Cloning, Molecular, LEUCINE-ZIPPER, Glutathione Transferase, Cell Nucleus, Microscopy, Confocal, Base Sequence, RECOGNITION, Intracellular Signaling Peptides and Proteins, Nuclear Proteins, RING FINGER, Blotting, Northern, GENE, FAMILY, Microscopy, Fluorescence, association in vivo, SIGNALING PROTEINS, MOTIF, Carrier Proteins, Dimerization, HeLa Cells
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