Ebola virus VP35 induces high-level production of recombinant TPL-2–ABIN-2–NF-κB1 p105 complex in co-transfected HEK-293 cells
Copyright policy )Ebola virus VP35 induces high-level production of recombinant TPL-2–ABIN-2–NF-κB1 p105 complex in co-transfected HEK-293 cells
Activation of PKR (double-stranded-RNA-dependent protein kinase) by DNA plasmids decreases translation, and limits the amount of recombinant protein produced by transiently transfected HEK (human embryonic kidney)-293 cells. Co-expression with Ebola virus VP35 (virus protein 35), which blocked plasmid activation of PKR, substantially increased production of recombinant TPL-2 (tumour progression locus 2)–ABIN-2 [A20-binding inhibitor of NF-κB (nuclear factor κB) 2]–NF-κB1 p105 complex. VP35 also increased expression of other co-transfected proteins, suggesting that VP35 could be employed generally to boost recombinant protein production by HEK-293 cells.
- Cancer Research UK Scotland Institute United Kingdom
- Cancer Research UK United Kingdom
- Boston University United States
- National Institute for Medical Research United Kingdom
- Boston College United States
NF-kappa B p50 Subunit, Accelerated Publication, Ebolavirus, MAP Kinase Kinase Kinases, Transfection, Recombinant Proteins, Up-Regulation, HEK293 Cells, Multiprotein Complexes, Proto-Oncogene Proteins, Humans, Viral Regulatory and Accessory Proteins, Adaptor Proteins, Signal Transducing
NF-kappa B p50 Subunit, Accelerated Publication, Ebolavirus, MAP Kinase Kinase Kinases, Transfection, Recombinant Proteins, Up-Regulation, HEK293 Cells, Multiprotein Complexes, Proto-Oncogene Proteins, Humans, Viral Regulatory and Accessory Proteins, Adaptor Proteins, Signal Transducing
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