Gene expression profiling identifies FKBP39 as an inhibitor of autophagy in larval Drosophila fat body
Copyright policy )Gene expression profiling identifies FKBP39 as an inhibitor of autophagy in larval Drosophila fat body
In Drosophila, the fat body undergoes a massive burst of autophagy at the end of larval development in preparation for the pupal transition. To identify genes involved in this process, we carried out a microarray analysis. We found that mRNA levels of the homologs of Atg8, the coat protein of early autophagic structures, and lysosomal hydrolases were upregulated, consistent with previous results. Genes encoding mitochondrial proteins and many chaperones were downregulated, including the inhibitor of eIF2alpha kinases and the peptidyl-prolyl cis-trans isomerase FK506-binding protein of 39 kDa (FKBP39). Genetic manipulation of FKBP39 expression had a significant effect on autophagy, potentially through modulation of the transcription factor Foxo. Accordingly, we found that Foxo mutants cannot properly undergo autophagy in response to starvation, and that overexpression of Foxo induces autophagy.
QH3011 Biochemistry / biokémia, QH3015 Molecular biology / molekuláris biológia, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Fat Body, PTEN Phosphohydrolase, Gene Expression Regulation, Developmental, Forkhead Transcription Factors, Tacrolimus Binding Proteins, Larva, Mutation, Autophagy, Animals, Drosophila Proteins, Drosophila, Oligonucleotide Array Sequence Analysis
QH3011 Biochemistry / biokémia, QH3015 Molecular biology / molekuláris biológia, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Fat Body, PTEN Phosphohydrolase, Gene Expression Regulation, Developmental, Forkhead Transcription Factors, Tacrolimus Binding Proteins, Larva, Mutation, Autophagy, Animals, Drosophila Proteins, Drosophila, Oligonucleotide Array Sequence Analysis
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