Neurod6 expression defines new retinal amacrine cell subtypes and regulates their fate
Copyright policy )Neurod6 expression defines new retinal amacrine cell subtypes and regulates their fate
Most regions of the CNS contain many subtypes of inhibitory interneurons with specialized roles in circuit function. In the mammalian retina, the ∼30 subtypes of inhibitory interneurons called amacrine cells (ACs) are generally divided into two groups: wide/medium-field GABAergic ACs and narrow-field glycinergic ACs, which mediate lateral and vertical interactions, respectively, within the inner plexiform layer. We used expression profiling and mouse transgenic lines to identify and characterize two closely related narrow-field AC subtypes. Both arise postnatally and one is neither glycinergic nor GABAergic (nGnG). Two transcription factors selectively expressed by these subtypes, Neurod6 and special AT-rich-sequence-binding protein 2 (Satb2), regulate a postmitotic cell fate choice between these subtypes. Satb2 induces Neurod6, which persists in nGnG ACs and promotes their fate but is downregulated in the related glycinergic AC subtype. Our results support the view that cell fate decisions made in progenitors and their progeny act together to diversify ACs.
- Harvard University United States
Glutamate Decarboxylase, Glycine, Gene Expression Regulation, Developmental, Mice, Transgenic, Nerve Tissue Proteins, Matrix Attachment Region Binding Proteins, In Vitro Techniques, Embryo, Mammalian, Article, Retina, Mice, Inbred C57BL, Luminescent Proteins, Mice, Amacrine Cells, Electroporation, Animals, Newborn, Bromodeoxyuridine, Glycine Plasma Membrane Transport Proteins, Basic Helix-Loop-Helix Transcription Factors, Animals, Macaca
Glutamate Decarboxylase, Glycine, Gene Expression Regulation, Developmental, Mice, Transgenic, Nerve Tissue Proteins, Matrix Attachment Region Binding Proteins, In Vitro Techniques, Embryo, Mammalian, Article, Retina, Mice, Inbred C57BL, Luminescent Proteins, Mice, Amacrine Cells, Electroporation, Animals, Newborn, Bromodeoxyuridine, Glycine Plasma Membrane Transport Proteins, Basic Helix-Loop-Helix Transcription Factors, Animals, Macaca
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