Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster
Copyright policy )Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster
The conditional expression of hairpin constructs in Drosophila melanogaster has emerged in recent years as a method of choice in functional genomic studies. To date, upstream activating site-driven RNA interference constructs have been inserted into the genome randomly using P-element-mediated transformation, which can result in false negatives due to variable expression. To avoid this problem, we have developed a transgenic RNA interference vector based on the phiC31 site-specific integration method.
- University of Massachusetts Amherst United States
- Massachusetts General Hospital United States
- Howard Hughes Medical Institute United States
- Janelia Research Campus United States
- Harvard University United States
Research Subject Categories::NATURAL SCIENCES::Chemistry::Biochemistry::Functional genomics, 570, Tools: Genetic Engineering, Genetic Vectors, 500, Gypsy Insulator, UAS-Luciferase, Notch RNAi, Drosophila melanogaster, Gene Targeting, Animals, Drosophila Proteins, RNA Interference, AttP sites, Biology, Biotechnology
Research Subject Categories::NATURAL SCIENCES::Chemistry::Biochemistry::Functional genomics, 570, Tools: Genetic Engineering, Genetic Vectors, 500, Gypsy Insulator, UAS-Luciferase, Notch RNAi, Drosophila melanogaster, Gene Targeting, Animals, Drosophila Proteins, RNA Interference, AttP sites, Biology, Biotechnology
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