A genome-scale shRNA resource for transgenic RNAi in Drosophila
Copyright policy )A genome-scale shRNA resource for transgenic RNAi in Drosophila
Existing transgenic RNAi resources in Drosophila melanogaster based on long double-stranded hairpin RNAs are powerful tools for functional studies, but they are ineffective in gene knockdown during oogenesis, an important model system for the study of many biological questions. We show that shRNAs, modeled on an endogenous microRNA, are extremely effective at silencing gene expression during oogenesis. We also describe our progress toward building a genome-wide shRNA resource.
- Tsinghua University China (People's Republic of)
- Massachusetts General Hospital United States
- Sanford Burnham Prebys Medical Discovery Institute United States
- Harvard Medical School United States
- Tsinghua University China (People's Republic of)
Research Subject Categories::NATURAL SCIENCES::Chemistry::Biochemistry::Functional genomics, 570, Base Sequence, Genetic Vectors, Genome, Insect, Life Sciences, Animals, Genetically Modified, MicroRNAs, Drosophila melanogaster, Oogenesis, Genetic Techniques, Gene Knockdown Techniques, Animals, Female, RNA Interference, RNA, Small Interfering, Biology, DNA Primers
Research Subject Categories::NATURAL SCIENCES::Chemistry::Biochemistry::Functional genomics, 570, Base Sequence, Genetic Vectors, Genome, Insect, Life Sciences, Animals, Genetically Modified, MicroRNAs, Drosophila melanogaster, Oogenesis, Genetic Techniques, Gene Knockdown Techniques, Animals, Female, RNA Interference, RNA, Small Interfering, Biology, DNA Primers
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