Crystal structure at 1.92 Å resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin
Copyright policy )Crystal structure at 1.92 Å resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin
The crystal structure of the RNA-binding domain of the small nuclear ribonucleoprotein U1A bound to a 21-nucleotide RNA hairpin has been determined at 1.92 A resolution. The ten-nucleotide RNA loop binds to the surface of the beta-sheet as an open structure, and the AUUGCAC sequence of the loop interacts extensively with the conserved RNP1 and RNP2 motifs and the C-terminal extension of the RNP domain. These interactions include stacking of RNA bases with aromatic side chains of proteins and many direct and water-mediated hydrogen bonds. The structure reveals the stereochemical basis for sequence-specific RNA recognition by the RNP domain.
- European Molecular Biology Laboratory Germany
- Medical Research Council United Kingdom
- European Bioinformatics Institute United Kingdom
- University of Leeds United Kingdom
- MRC Laboratory of Molecular Biology United Kingdom
Models, Molecular, Binding Sites, Base Sequence, Molecular Sequence Data, RNA-Binding Proteins, Hydrogen Bonding, Crystallography, X-Ray, Protein Structure, Secondary, Protein Structure, Tertiary, Ribonucleoprotein, U1 Small Nuclear, RNA, Small Nuclear, Spliceosomes, Nucleic Acid Conformation, Amino Acid Sequence
Models, Molecular, Binding Sites, Base Sequence, Molecular Sequence Data, RNA-Binding Proteins, Hydrogen Bonding, Crystallography, X-Ray, Protein Structure, Secondary, Protein Structure, Tertiary, Ribonucleoprotein, U1 Small Nuclear, RNA, Small Nuclear, Spliceosomes, Nucleic Acid Conformation, Amino Acid Sequence
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).887 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 0.1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 0.1%
Citations provided by BIP!Popularity provided by BIP!