Expression of insulin-like growth factors IGF-I and IGF-II, and their receptors during the growth and megakaryocytic differentiation of K562 cells
Copyright policy )Expression of insulin-like growth factors IGF-I and IGF-II, and their receptors during the growth and megakaryocytic differentiation of K562 cells
Insulin-like growth factors (IGFs) I and II are critical regulators of cell proliferation and differentiation and most of the growth promoting properties of both ligands are mediated by IGF-I receptor (IGF-IR). In the present study we have investigated the role of IGFs in K562 cell line during normal growth and 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-induced megakaryocytic differentiation. Abundant expression of IGF-I, IGF-II and IGF-IR was demonstrated in resting cells and exogenous IGF-I and IGF-II increased 3H-thymidine incorporation in a dose dependent manner. In contrast, we found that basal growth of the cells was inhibited by using anti-IGF-IR mAb. Furthermore, also IGF-I and IGF-II induced DNA synthesis was significantly suppressed by anti-IGF-IR mAb. During megakaryocytic differentiation, expression of IGF-IR increased during first 12h, but after that the expression started to decrease together with IGF-I. Taken together, our data suggest that autocrine production of IGF-I and IGF-II may via IGF-IR play a significant role in the growth and megakaryocytic differentiation of K562 cells.
- University of Helsinki Finland
- Helsinki University Hospital Finland
DNA Replication, Gene Expression Regulation, Leukemic, Antibodies, Monoclonal, Cell Differentiation, Receptor, IGF Type 2, Neoplasm Proteins, Receptor, IGF Type 1, Autocrine Communication, Mice, Protein Subunits, Insulin-Like Growth Factor II, Animals, Humans, Tetradecanoylphorbol Acetate, Insulin-Like Growth Factor I, K562 Cells, Megakaryocytes
DNA Replication, Gene Expression Regulation, Leukemic, Antibodies, Monoclonal, Cell Differentiation, Receptor, IGF Type 2, Neoplasm Proteins, Receptor, IGF Type 1, Autocrine Communication, Mice, Protein Subunits, Insulin-Like Growth Factor II, Animals, Humans, Tetradecanoylphorbol Acetate, Insulin-Like Growth Factor I, K562 Cells, Megakaryocytes
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