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A Role for the Segment Polarity Gene shaggy/GSK-3 in the Drosophila Circadian Clock

descriptionPublicationkeyboard_double_arrow_right Article 01 Jun 2001 English Publisher:Elsevier BVJournal:Cell, volume 105, pages 769-779 (issn: 0092-8674,

Authors: Sebastian Martinek; Armen S. Manoukian; Susan Inonog; Michael W. Young;

doi: 10.1016/s0092-8674(01)00383-x

pmid: 11440719

A Role for the Segment Polarity Gene shaggy/GSK-3 in the Drosophila Circadian Clock

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Abstract

Tissue-specific overexpression of the glycogen synthase kinase-3 (GSK-3) ortholog shaggy (sgg) shortens the period of the Drosophila circadian locomotor activity cycle. The short period phenotype was attributed to premature nuclear translocation of the PERIOD/TIMELESS heterodimer. Reducing SGG/GSK-3 activity lengthens period, demonstrating an intrinsic role for the kinase in circadian rhythmicity. Lowered sgg activity decreased TIMELESS phosphorylation, and it was found that GSK-3 beta specifically phosphorylates TIMELESS in vitro. Overexpression of sgg in vivo converts hypophosphorylated TIMELESS to a hyperphosphorylated protein whose electrophoretic mobility, and light and phosphatase sensitivity, are indistinguishable from the rhythmically produced hyperphosphorylated TIMELESS of wild-type flies. Our results indicate a role for SGG/GSK-3 in TIMELESS phosphorylation and in the regulated nuclear translocation of the PERIOD/TIMELESS heterodimer.

Related Organizations

Ontario Institute for Cancer Research
Canada
Rockefeller University
United States

Keywords

Cell Nucleus, Biochemistry, Genetics and Molecular Biology(all), Recombinant Fusion Proteins, Immunoblotting, Active Transport, Cell Nucleus, Nuclear Proteins, Period Circadian Proteins, Motor Activity, Protein Serine-Threonine Kinases, Circadian Rhythm, Glycogen Synthase Kinase 3, Drosophila melanogaster, Microscopy, Fluorescence, Biological Clocks, Animals, Drosophila Proteins, Insect Proteins, RNA, Phosphorylation, Dimerization

20 Research products, page 1 of 2

SGG
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SGG
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Shaggy, isoform R
2017IsRelatedTo
Gal4
2017IsRelatedTo
Shaggy, isoform J
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Shaggy, isoform Q
2017IsRelatedTo
Shaggy, isoform K
2017IsRelatedTo
SGG
2017IsRelatedTo
Shaggy, isoform M
2017IsRelatedTo
SGG
2017IsRelatedTo

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