Yeast HOG1 MAP Kinase Cascade Is Regulated by a Multistep Phosphorelay Mechanism in the SLN1–YPD1–SSK1 “Two-Component” Osmosensor
Copyright policy )Yeast HOG1 MAP Kinase Cascade Is Regulated by a Multistep Phosphorelay Mechanism in the SLN1–YPD1–SSK1 “Two-Component” Osmosensor
An osmosensing mechanism in the budding yeast (Saccharomyces cerevisiae) involves both a two-component signal transducer (Sln1p, Ypd1p and Ssk1p) and a MAP kinase cascade (Ssk2p/Ssk22p, Pbs2p, and Hog1p). The transmembrane protein Sln1p contains an extracellular sensor domain and cytoplasmic histidine kinase and receiver domains, whereas the cytoplasmic protein Ssk1p contains a receiver domain. Ypd1p binds to both Sln1p and Ssk1p and mediates the multistep phosphotransfer reaction (phosphorelay). This phosphorelay system is initiated by the autophosphorylation of Sln1p at His576. This phosphate is then sequentially transferred to Sln1p-Asp-1144, then to Ypd1p-His64, and finally to Ssk1p-Asp554. We propose that the multistep phosphorelay mechanism is a universal signal transduction apparatus utilized both in prokaryotes and eukaryotes.
- Dana-Farber Cancer Institute United States
- Harvard University United States
Aspartic Acid, Binding Sites, Saccharomyces cerevisiae Proteins, Biochemistry, Genetics and Molecular Biology(all), Genes, Fungal, Molecular Sequence Data, Intracellular Signaling Peptides and Proteins, Saccharomyces cerevisiae, Water-Electrolyte Balance, DNA-Binding Proteins, Fungal Proteins, Phenotype, Calcium-Calmodulin-Dependent Protein Kinases, Histidine, Amino Acid Sequence, Cloning, Molecular, Mitogen-Activated Protein Kinases, Phosphorylation, Protein Kinases, Signal Transduction
Aspartic Acid, Binding Sites, Saccharomyces cerevisiae Proteins, Biochemistry, Genetics and Molecular Biology(all), Genes, Fungal, Molecular Sequence Data, Intracellular Signaling Peptides and Proteins, Saccharomyces cerevisiae, Water-Electrolyte Balance, DNA-Binding Proteins, Fungal Proteins, Phenotype, Calcium-Calmodulin-Dependent Protein Kinases, Histidine, Amino Acid Sequence, Cloning, Molecular, Mitogen-Activated Protein Kinases, Phosphorylation, Protein Kinases, Signal Transduction
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).799 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 0.1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 0.1%
Citations provided by BIP!Popularity provided by BIP!