Rac is involved in the interkinetic nuclear migration of cortical progenitor cells
Copyright policy )Rac is involved in the interkinetic nuclear migration of cortical progenitor cells
The small GTPase Rac regulates neuronal behavior, but whether it also functions in neural progenitor cells has not yet been explored. Here we report that Rac contributes to the regulation of nuclear migration in neocortical progenitor cells. Rac1 is expressed by progenitor cells in a unique spatiotemporal pattern. Cross-sectional immunohistochemical examination revealed intense Rac1 immunoreactivity at the ventricular surface. Similar staining patterns were obtained by immunofluorescence for a Rac-activator, Tiam1, and by reactions to detect the GTP-bound (active) form of Rac. En face inspection of the ventricular surface revealed that apical Rac1 localization was most frequent in M-phase cells, and the endfeet of cells in other cell cycle phases also showed apical Rac1 distribution at lower frequencies. To ask whether progenitor cell behavior prior to and during M phase is Rac-dependent, we monitored individual DiI-labeled progenitor cells live in the presence of a Rac inhibitor, NSC23766. We observed significantly retarded adventricular nuclear migration, as well as cytokinesis failures. Similar inhibitory effects were obtained by forced expression of a dominant-negative Rac1. These results suggest that Rac may play a role in interkinetic nuclear migration in the developing mouse brain.
- Nagoya University Japan
- Mie University Japan
- Chubu University Japan
- Keio University Japan
- S. C. Institute of Health Science and Research Japan
Cerebral Cortex, Neurons, Mice, Inbred ICR, Neurogenesis, Cell Cycle, Green Fluorescent Proteins, Age Factors, Gene Expression Regulation, Developmental, Embryo, Mammalian, Cerebral Ventricles, Mice, Electroporation, Organ Culture Techniques, Cell Movement, Pregnancy, Aminoquinolines, Animals, Guanine Nucleotide Exchange Factors, Female, Amino Acids
Cerebral Cortex, Neurons, Mice, Inbred ICR, Neurogenesis, Cell Cycle, Green Fluorescent Proteins, Age Factors, Gene Expression Regulation, Developmental, Embryo, Mammalian, Cerebral Ventricles, Mice, Electroporation, Organ Culture Techniques, Cell Movement, Pregnancy, Aminoquinolines, Animals, Guanine Nucleotide Exchange Factors, Female, Amino Acids
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