OsNF-YB1, a rice endosperm-specific gene, is essential for cell proliferation in endosperm development
Copyright policy )OsNF-YB1, a rice endosperm-specific gene, is essential for cell proliferation in endosperm development
Cell cycle regulators are crucial for normal endosperm development and seed size determination. However, how the cell cycle related genes regulate endosperm development remains unclear. In this study, we reported a rice Nuclear Factor Y (NF-Y) gene OsNF-YB1, which was also identified as an endosperm-specific gene. Transcriptional profiling and promoter analysis revealed that OsNF-YB1 was highly expressed at the early stages of rice endosperm development (5-7 DAP, days after pollination). Repression of OsNF-YB1 resulted in differential expression of the genes in cell cycle pathway, which caused abnormal seeds with defected embryo and endosperm. Basic cytological analysis demonstrated that the reduced endosperm cell numbers disintegrated with the development of those abnormal seeds in OsNF-YB1 RNAi plants. Taken together, these results suggested that the endosperm-specific gene OsNF-YB1 might be a cell cycle regulator and played a role in maintaining the endosperm cell proliferation.
- Huazhong Agricultural University China (People's Republic of)
Time Factors, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Cell Cycle, Green Fluorescent Proteins, Gene Expression Regulation, Developmental, Oryza, Plants, Genetically Modified, Endosperm, Microscopy, Fluorescence, Gene Expression Regulation, Plant, RNA Interference, Promoter Regions, Genetic, Phylogeny, Cell Proliferation, Plant Proteins, Signal Transduction
Time Factors, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Cell Cycle, Green Fluorescent Proteins, Gene Expression Regulation, Developmental, Oryza, Plants, Genetically Modified, Endosperm, Microscopy, Fluorescence, Gene Expression Regulation, Plant, RNA Interference, Promoter Regions, Genetic, Phylogeny, Cell Proliferation, Plant Proteins, Signal Transduction
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