Protein kinase Cθ gene expression is oppositely regulated by GCN5 and EBF1 in immature B cells
Copyright policy )Protein kinase Cθ gene expression is oppositely regulated by GCN5 and EBF1 in immature B cells
In this study, we revealed that GCN5 and early B cell factor 1 (EBF1) participate in regulation of protein kinase Cθ (PKCθ) gene expression in an opposite manner in immature B cells. GCN5‐deficiency in DT40 caused drastic down‐regulation of transcription of PKCθ. In contrast, EBF1‐deficiency brought about remarkable up‐regulation of that of PKCθ, and re‐expression of EBF1 dramatically suppressed transcription of PKCθ. Chromatin immunoprecipitation assay revealed that GCN5 binds to the 5′‐flanking region of the chicken PKCθ gene and acetylates histone H3, and EBF1 binds to the 5′‐flanking region of the gene surrounding putative EBF1 binding motifs.
- University of Tokyo Japan
- University of Miyazaki Japan
- Kawasaki Medical School Japan
- Kawasaki Medical School Japan
5' Flanking Region, Molecular Sequence Data, Down-Regulation, Avian Proteins, Histones, Early B cell factor 1, Animals, RNA, Messenger, Cells, Cultured, Protein Kinase C, Histone Acetyltransferases, B-Lymphocytes, Binding Sites, Base Sequence, DT40, Gene targeting, Acetylation, PKCθ, Trans-Activators, Enzyme Repression, Chickens, Protein Processing, Post-Translational, GCN5, Protein Binding
5' Flanking Region, Molecular Sequence Data, Down-Regulation, Avian Proteins, Histones, Early B cell factor 1, Animals, RNA, Messenger, Cells, Cultured, Protein Kinase C, Histone Acetyltransferases, B-Lymphocytes, Binding Sites, Base Sequence, DT40, Gene targeting, Acetylation, PKCθ, Trans-Activators, Enzyme Repression, Chickens, Protein Processing, Post-Translational, GCN5, Protein Binding
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